猪肿瘤坏死因子α(TNFa)酶联免疫吸附检测试剂盒

TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

市场价:¥1960.00
商城价:¥1470.00
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商品品牌:
ELK Biotechnology
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    基本信息
    商品名称 猪肿瘤坏死因子α(TNFa)酶联免疫吸附检测试剂盒
    别名 TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
    品牌 ELK Biotechnology
    货号 ELK2407
    Reactivity
    Assay Type Sandwich
    Sensitivity 6.5 pg/mL
    standard 1000 pg/mL
    Detection range 15.63-1000 pg/mL
    Sample type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    Assay length 3.5h
    Research Area Cytokine;Tumor immunity;Infection immunity;
    Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.

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